Cp-thionin-1 (Cp-thionin I) (Gamma-thionin I)

id PHYT00005
Name Cp-thionin-1 (Cp-thionin I) (Gamma-thionin I)
Gene Unidentified
Family Defensin family
Plant Vigna unguiculata (Cowpea)
Taxonomy Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons; rosids; eurosids I; Fabales; Fabaceae; Papilionoideae; Phaseoleae; Vigna;
Activity Unknown
Target organisms Unknown
Description Function:
Inhibits trypsin but not chymotrypsin.
Similarity:
Belongs to the plant defensin family.
Mass spectrometry:
Mass=5229.03; Method=MALDI; Range=1-47; Source=PubMed:12112698;
Subunit:
Monomer and homodimer.
[1]
PROTEIN SEQUENCE, FUNCTION, SUBUNIT, MASS SPECTROMETRY, AND3D-STRUCTURE MODELING.
MEDLINE=22107283;PubMed=12112698;DOI=10.1002/prot.10142
Melo F.R., Rigden D.J., Franco O.L., Mello L.V., Ary M.B.,Grossi de Sa M.F., Bloch C. Jr.
"Inhibition of trypsin by cowpea thionin: characterization, molecularmodeling, and docking.", Proteins 48:311-319(2002).


Formula C210 H333 N75 O63 S8
Absent amino acids IMPWY
Common amino acids C
Mass (Da) 5191.68
Net charge +7
Isoelectric point 8.61
Basic residues 10
Acidic residues 3
Hydrophobic residues 10
Polar residues 23
Aliphatic residues 4
Tiny residues 12
Boman Index -122.8
Hydropathy Index -0.46
Aliphatic Index 33.19
Instability Index 67.75 (unstable)
Half Life Mammalian : 1 hour
Yeast : 2 min
E. coli : 2 min
Extinction Coefficient 500 M-1 cm-1
Absorbance 280nm 10.87
Composition

Databases

Uniprot:

PDB Structure:

Unknown

Other databases:

HSSP P20230

Sequence:

........10 ........20 ........30 
         |          |          | 
RVCESQSHGFKGACTGDHNCALVCRNEGFS
GGNCRGFRRRCFCTLKC

Method of sequencing:

Direct sequencing