Ah-AMP1

id PHYT00001
Name Ah-AMP1
Gene Unidentified
Family Defensin family
Plant Aesculus hippocastanum (Horse chestnut)
Taxonomy Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons; rosids; eurosids II; Sapindales; Sapindaceae; Aesculus;
Activity Antibacterial, Antifungal
Target organisms Gram-positive bacteria: Bacillus subtilis (IC50= 100g/mL).
Fungi: MA/MA+ (IC50 g/mL) Botrytis cinerea (25, >100), Cladosporium sphaerospermum (0.5, 12), Fusarium culmorum (12, >100), Leptosphaeria maculans (0.5, 6), Penicillium digitatum (6, 25), Trichoderma viride (>100, >100), Septoria tritiei (0.5, 1.5), Verticilium albo-atrum (6, >100).
NOTE! MA+ = Medium A supplemented with 1 mM CaCl2 and 50 mM KCl.
Description
[1]
STRUCTURE BY NMR.
MEDLINE=20058644;PubMed=10591099;DOI=10.1002/(SICI)1097-0134(19991115)37:3<388::AID-PROT7>3.3.CO
Fant F., Vranken W.F., Borremans F.A.
"The three-dimensional solution structure of Aesculus hippocastanumantimicrobial protein 1 determined by 1H nuclear magnetic resonance.", Proteins 37:388-403(1999).


[2]
PROTEIN SEQUENCE.
MEDLINE=95354848;PubMed=7628617;DOI=10.1016/0014-5793(95)00666-W
Osborn R.W., De Samblanx G.W., Thevissen K., Goderis I., Torrekens S.,Van Leuven F., Attenborough S., Rees S.B., Broekaert W.F.
"Isolation and characterisation of plant defensins from seeds ofAsteraceae, Fabaceae, Hippocastanaceae and Saxifragaceae.", FEBS Lett. 368:257-262(1995).


Formula C245 H355 N79 O75 S8
Absent amino acids IMV
Common amino acids C
Mass (Da) 5882.33
Net charge +5
Isoelectric point 7.71
Basic residues 10
Acidic residues 5
Hydrophobic residues 9
Polar residues 22
Aliphatic residues 1
Tiny residues 9
Boman Index -137.49
Hydropathy Index -1.17
Aliphatic Index 13.8
Instability Index 40.35 (unstable)
Half Life Mammalian : 5.5 hour
Yeast : 3 min
E. coli : 2 min
Extinction Coefficient 18490 M-1 cm-1
Absorbance 280nm 377.35
Composition

Databases

Uniprot:

PDB Structure:

1BK8 resolved by NMR

Other databases:

PIR S66218

Sequence:

........10 ........20 ........30 
         |          |          | 
LCNERPSQTWSGNCGNTAHCDKQCQDWEKA
SHGACHKRENHWKCFCYFNC

Method of sequencing:

Direct sequencing